Agilent FTIR spectroscopic imaging technology has helped enable a new process for precisely marking the boundaries of cancerous tissues.
Normally, a tissue sample is chemically stained, then a histopathologist manually annotates the cancerous boundaries. This process is prone to inaccuracies, due to its manual and subjective nature. It also requires an experienced histopathologist.
German researchers have developed a new and novel process that uses Agilent FTIR imaging technology. This process generates images based on the molecular make-up of the sample. Computer models automatically and objectively determine the cancerous/non-cancerous boundary.
The FTIR imaging approach is stain-free and non-destructive. It provides a more “pure” cancer sample that can subsequently be analyzed at the molecular level, including proteomics, genomics and metabolomics. Proteomics can eventually help identify cancer biomarkers.
The researchers analyzed tissue samples from diffuse malignant mesothelioma, a type of lung cancer. They found biomarkers that enabled them to distinguish between two subtypes – sarcomatoid and epitheloid – by examining more than 2,000 related proteins. These biomarkers can potentially determine the likely therapeutic response of the cancer to certain treatments.
The researchers intend to apply their method to other types of cancer, to identify new biomarkers. They hope their work will lead to future diagnostic tests that are simple, precise, predictive and non-invasive.
FTIR stands for “Fourier Transform Infrared.” (Fourier was a French mathematician.) FTIR spectroscopy provides a total molecular chemical signature of a sample, by analyzing it over a broad infrared spectral range.
For Research Use Only. Not for use in diagnostic procedures.
Thanks to Dr. Mustafa Kansiz, Agilent Infrared Microscopy and Imaging Product Manager, for his help with today’s post!
For more information go to:
- Spatial and molecular resolution of diffuse malignant mesothelioma heterogeneity by integrating label-free FTIR imaging, laser capture microdissection and proteomics
- New process for identifying biomarkers established (Ruhr-Universität Bochum)
- Protein Research Unit Ruhr Within Europe (PURE)
- Emerging life science applications of FTIR Imaging: Providing spatially resolved molecular information in disease research (Access Agilent)
- Agilent supports GC/MS with fast, accurate FTIR screening of phthalates in toys (Access Agilent)
- Agilent FTIR solutions for food authenticity (Access Agilent)
- Agilent Advanced FTIR Spectroscopy Instruments and Supplies
- Agilent FTIR Imaging: The Power of a Synchrotron
- Agilent Sample Quality Control Solutions for Cell and Tissue Samples (PDF)